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  The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles.

Matkovic, T., Siebert, M., Knoche, E., Depner, H., Mertel, S., Owald, D., et al. (2013). The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles. Journal of Cell Biology, 202(4), 667-683. doi:10.1083/jcb.201301072.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-500B-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3676-8
Genre: Journal Article

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 Creators:
Matkovic, T., Author
Siebert, M., Author
Knoche, E., Author
Depner, H., Author
Mertel, S., Author
Owald, D., Author
Schmidt, M., Author
Thomas, U., Author
Sickmann, D., Author
Kamin, D.1, Author              
Hell, S. W.1, Author              
Bürger, J., Author
Hollmann, C., Author
Mielke, T., Author
Wichmann, C., Author
Sigrist, S. J., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Synaptic vesicles (SVs) fuse at a specialized membrane domain called the active zone (AZ), covered by a conserved cytomatrix. How exactly cytomatrix components intersect with SV release remains insufficiently understood. We showed previously that loss of the Drosophila melanogaster ELKS family protein Bruchpilot (BRP) eliminates the cytomatrix (T bar) and declusters Ca2+ channels. In this paper, we explored additional functions of the cytomatrix, starting with the biochemical identification of two BRP isoforms. Both isoforms alternated in a circular array and were important for proper T-bar formation. Basal transmission was decreased in isoform-specific mutants, which we attributed to a reduction in the size of the readily releasable pool (RRP) of SVs. We also found a corresponding reduction in the number of SVs docked close to the remaining cytomatrix. We propose that the macromolecular architecture created by the alternating pattern of the BRP isoforms determines the number of Ca2+ channel-coupled SV release slots available per AZ and thereby sets the size of the RRP.

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Language(s): eng - English
 Dates: 2013-08-19
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201301072
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Title: Journal of Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 202 (4) Sequence Number: - Start / End Page: 667 - 683 Identifier: -