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  Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins.

Chevelkov, V., Shi, C., Fasshuber, H. K., Becker, S., & Lange, A. (2013). Efficient band-selective homonuclear CO-CA cross-polarization in protonated proteins. Journal of Biomolecular NMR, 56(4), 303-311. doi:10.1007/s10858-013-9767-1.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-55AB-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DD1A-3
Genre: Journal Article

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 Creators:
Chevelkov, V.1, Author              
Shi, C., Author
Fasshuber, H. K.1, Author              
Becker, S.2, Author              
Lange, A.1, Author              
Affiliations:
1Research Group of Solid-State NMR, MPI for Biophysical Chemistry, Max Planck Society, ou_persistent35              
2Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Free keywords: Solid-state magic-angle spinning NMR; Homonuclear recoupling; Protein resonance assignment; Microcrystalline ubiquitin
 Abstract: Previously introduced for highly deuterated proteins, band-selective magnetization transfer between CO and CA spins by dipolar-based homonuclear cross polarization is applied here to a protonated protein. Robust and efficient recoupling is achieved when the sum of effective radio-frequency fields on CO and CA resonances equals two times the spinning rate, yielding up to 33 % of magnetization transfer efficiency in protonated ubiquitin. The approach is designed for moderate magic-angle spinning rates and high external magnetic fields when the isotropic chemical shift difference of CO and CA considerably exceeds the spinning rate. This method has been implemented in N(i)CO(i-1)CA(i-1) and CA(i)(N-i)CO(i-1)CA(i-1) two-dimensional interresidual correlation experiments for fast and efficient resonance assignment of ubiquitin by solid-state NMR spectroscopy.

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Language(s): eng - English
 Dates: 2013-08-082013-08
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10858-013-9767-1
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 56 (4) Sequence Number: - Start / End Page: 303 - 311 Identifier: -