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  Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81

Bhogaraju, S., Cajanek, L., Fort, C., Blisnick, T., Weber, K., Taschner, M., et al. (2013). Molecular Basis of Tubulin Transport Within the Cilium by IFT74 and IFT81. SCIENCE, 341(6149), 1009-1012. doi:10.1126/science.1240985.

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 Creators:
Bhogaraju, Sagar1, Author              
Cajanek, Lukas2, Author
Fort, Cecile2, Author
Blisnick, Thierry2, Author
Weber, Kristina3, Author              
Taschner, Michael1, Author              
Mizuno, Naoko4, Author              
Lamla, Stefan2, Author
Bastin, Philippe2, Author
Nigg, Erich A.2, Author
Lorentzen, Esben1, Author              
Affiliations:
1Lorentzen, Esben / Intraflagellar Transport, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565157              
2external, ou_persistent22              
3Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
4Mizuno, Naoko / Cellular and Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_1688137              

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Free keywords: FLAGELLAR LENGTH CONTROL; INTRAFLAGELLAR TRANSPORT; CHLAMYDOMONAS; PROTEIN; COMPLEX; TURNOVER; CILIOGENESIS; MOTILITY; DYNEIN; CELLS
 Abstract: Intraflagellar transport (IFT) of ciliary precursors such as tubulin from the cytoplasm to the ciliary tip is involved in the construction of the cilium, a hairlike organelle found on most eukaryotic cells. However, the molecular mechanisms of IFT are poorly understood. Here, we found that the two core IFT proteins IFT74 and IFT81 form a tubulin-binding module and mapped the interaction to a calponin homology domain of IFT81 and a highly basic domain in IFT74. Knockdown of IFT81 and rescue experiments with point mutants showed that tubulin binding by IFT81 was required for ciliogenesis in human cells.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000323652300045
DOI: 10.1126/science.1240985
 Degree: -

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Title: SCIENCE
Source Genre: Journal
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Publ. Info: 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA : AMER ASSOC ADVANCEMENT SCIENCE
Pages: - Volume / Issue: 341 (6149) Sequence Number: - Start / End Page: 1009 - 1012 Identifier: ISSN: 0036-8075