hide
Free keywords:
-
Abstract:
For many decades, it has been accepted that the resolution of a lens-based optical microscope is limited to about d =λ (2 NA) < 200 nm. The discovery in the 1990’s that elementary transitions between the states of a fluorophore can be used to eliminate the limiting role of diffraction has led to lens-based light microscopy concepts with resolution down to the nanometer scale1,2. Currently, all far-field fluorescence nanoscopy (superresolution) concepts that have found wider application share a common enabling element: they modulate the fluorescence capability of adjacent features such that they fluoresce sequentially3,4.