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  Reduced sleep-like quiescence in both hyperactive and hypoactive mutants of the Galphaq gene egl-30 during lethargus in Caenorhabditis elegans.

Schwarz, J., & Bringmann, H. (2013). Reduced sleep-like quiescence in both hyperactive and hypoactive mutants of the Galphaq gene egl-30 during lethargus in Caenorhabditis elegans. PLoS One, 8(9): e75853. doi:10.1371/journal.pone.0075853.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-7436-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CB64-E
Genre: Journal Article

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 Creators:
Schwarz, J.1, Author              
Bringmann, H.1, Author              
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1Research Group of Sleep and Waking, MPI for biophysical chemistry, Max Planck Society, ou_578607              

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 Abstract: Sleep-like states are characterized by massively reduced behavioral activity. Little is known about genetic control of sleep-like behavior. It is also not clear how general activity levels during wake-like behavior influence activity levels during sleep-like behavior. Mutations that increase wake-like activity are generally believed to also increase activity during sleep-like behavior and mutations that decrease wake-like activity are believed to have decreased activity during sleep-like behavior. We studied sleep-like behavior during lethargus in larvae of Caenorhabditis elegans. We looked through a small set of known mutants with altered activity levels. As expected, mutants with increased activity levels typically showed less sleep-like behavior. Among these hyperactive mutants was a gain-of-function mutant of the conserved heterotrimeric G protein subunit Galphaq gene egl-30. We found, however, that an unusual semidominant hypoactive mutant of egl-30 also had reduced sleep-like behavior. While movement was severely reduced and impaired in the semidominant egl-30 mutant, sleep-like behavior was severely reduced: the semidominant egl-30 mutant lacked prolonged periods of complete immobility, reduced spontaneous neural activity less, and reduced responsiveness to stimulation less. egl-30 is a well-known regulator of behavior. Our results suggest that egl-30 controls not only general activity levels, but also differences between wake-like and sleep-like behavior.

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Language(s): eng - English
 Dates: 2013-09-20
 Publication Status: Published online
 Pages: 16
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pone.0075853
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Title: PLoS One
Source Genre: Journal
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Pages: - Volume / Issue: 8 (9) Sequence Number: e75853 Start / End Page: - Identifier: -