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  Erg Channel Is Critical in Controlling Cell Volume during Cell Cycle in Embryonic Stem Cells

Abdelhady, S., Kitambi, S. S., Lundin, V., Aufschnaiter, R., Sekyrova, P., Sinha, I., et al. (2013). Erg Channel Is Critical in Controlling Cell Volume during Cell Cycle in Embryonic Stem Cells. PLOS ONE, 8(8): e72409. doi:10.1371/journal.pone.0072409.

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 Creators:
Abdelhady, Shaimaa1, Author
Kitambi, Satish Srinivas1, Author
Lundin, Vanessa1, Author
Aufschnaiter, Roland2, Author              
Sekyrova, Petra1, Author
Sinha, Indranil1, Author
Lundgren, Kalle T.1, Author
Castelo-Branco, Goncalo1, Author
Linnarsson, Sten1, Author
Wedlich-Söldner, Roland2, Author              
Teixeira, Ana1, Author
Andang, Michael1, Author
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1external, ou_persistent22              
2Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              

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Free keywords: HERG K+ CHANNEL; RNA-SEQ; PROLIFERATION; DERIVATION; APOPTOSIS
 Abstract: The cell cycle progression in mouse embryonic stem cells (mESCs) is controlled by ion fluxes that alter cell volume [1]. This suggests that ion fluxes might control dynamic changes in morphology over the cell cycle, such as rounding up of the cell at mitosis. However, specific channels regulating such dynamic changes and the possible interactions with actomyosin complex have not been clearly identified. Following RNAseq transcriptome analysis of cell cycle sorted mESCs, we found that expression of the K+ ion channel Erg1 peaked in G1 cell cycle phase, which was confirmed by immunostaining. Inhibition of Erg channel activity caused loss of G1 phase cells via non-apoptotic cell death. Cells first lost the ability of membrane blebbing, a typical feature of cultured embryonic stem cells. Continued Erg inhibition further increased cell volume and the cell eventually ruptured. In addition, atomic force measurements on live cells revealed a decreased cortical stiffness after treatment, suggesting alterations in actomyosin organization. When the intracellular osmotic pressure was experimentally decreased by hypertonic solution or block of K+ ion import via the Na, K-ATPase, cell viability was restored and cells acquired normal volume and blebbing activity. Our results suggest that Erg channels have a critical function in K+ ion homeostasis of mESCs over the cell cycle, and that cell death following Erg inhibition is a consequence of the inability to regulate cell volume.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: PLOS ONE
Source Genre: Journal
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Publ. Info: 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA : PUBLIC LIBRARY SCIENCE
Pages: - Volume / Issue: 8 (8) Sequence Number: e72409 Start / End Page: - Identifier: ISSN: 1932-6203