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  Resolution enhancement of suspended microchannel resonators for weighing of biomolecular complexes in solution.

Modena, M. M., Wang, Y., Riedel, D., & Burg, T. P. (2014). Resolution enhancement of suspended microchannel resonators for weighing of biomolecular complexes in solution. Lab on a Chip, 14(2), 342-350. doi:10.1039/C3LC51058A.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-9A77-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-5B9C-6
Genre: Journal Article

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 Creators:
Modena, M. M.1, Author              
Wang, Y.1, Author              
Riedel, D.2, Author              
Burg, T. P.1, Author              
Affiliations:
1Research Group of Biological Micro- and Nanotechnology, MPI for biophysical chemistry, Max Planck Society, ou_578602              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Abstract: We introduce the use of correlation analysis to extend the dynamic range of Suspended Micro- and Nanochannel Resonator (SMR/SNR) mass sensors by over five orders of magnitude. This method can analyze populations of particles flowing through an embedded channel micromechanical resonator, even when the individual particle masses are far below the noise floor. To characterize the method, we measured the mass of polystyrene nanoparticles with 300 zg resolution. As an application, we monitored the time course of insulin amyloid formation from pre-fibrillar aggregates to mature fibrils of 15 MDa average mass. Results were compared with Thioflavin-T (ThT) assays and electron microscopy (EM). Mass measurements offer additional information over ThT during the fluorescent inaccessible lag period, and the average fibril dimensions calculated from the mass signal are in good accordance with EM. In the future, we envision that more detailed modeling will allow the computational deconvolution of multicomponent samples, enabling the mass spectrometric characterization of a variety of biomolecular complexes, small organelles, and nanoparticles in solution.

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Language(s): eng - English
 Dates: 2013-11-182014
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1039/C3LC51058A
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Title: Lab on a Chip
Source Genre: Journal
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Pages: - Volume / Issue: 14 (2) Sequence Number: - Start / End Page: 342 - 350 Identifier: -