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  Immunostimulatory activity of murine keratinocyte-derived exosomes.

Kotzerke, K., Mempel, M., Aung, T., Wulf, G. G., Urlaub, H., Wenzel, D., et al. (2013). Immunostimulatory activity of murine keratinocyte-derived exosomes. Experimental Dermatology, 22(10), 650-655. doi:10.1111/exd.12230.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-9AAB-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CB73-C
Genre: Journal Article

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1850975.pdf (Publisher version), 650KB
 
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 Creators:
Kotzerke, K., Author
Mempel, M., Author
Aung, T., Author
Wulf, G. G., Author
Urlaub, H.1, Author              
Wenzel, D.2, Author              
Schön, M. P., Author
Braun, A., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
2Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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Free keywords: exosomes; innate immunity; keratinocytes; skin
 Abstract: It has long been known that keratinocytes influence cutaneous immunity through secretion of soluble factors. Exosomes, small membrane vesicles of endocytotic origin, have been implicated in intercellular communication processes such as the transfer of tumor cell antigens and the activation of recipient dendritic cells (DC). However, little is known about immunomodulatory functions of keratinocyte-derived exosomes. To address this question, we analysed exosome secretion of the murine keratinocyte cell line MPEK under steady state as well as inflammatory conditions (+/- IFN). These exosomes were readily taken up by bone marrow-derived DC (BMDC) in vitro resulting in a matured phenotype, as evidenced by increased CD40 expression as well as by the production of large amounts of IL-6, IL-10 and IL-12. When the transfer of antigen-specific information through exosomes was investigated, it was found that keratinocytes took up antigen (ovalbumin) and transferred it to their exosomes. However, these antigen-harbouring exosomes failed to induce antigen-specific T cell responses via BMDC. Together, this novel biological function suggests that keratinocytes are able to direct unspecific immune processes but do not elicit specific immune responses.

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Language(s): eng - English
 Dates: 2013-09-232013-10
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1111/exd.12230
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Title: Experimental Dermatology
Source Genre: Journal
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Pages: - Volume / Issue: 22 (10) Sequence Number: - Start / End Page: 650 - 655 Identifier: -