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  NEW ENZYMATIC PROTECTING GROUP TECHNIQUES FOR THE CONSTRUCTION OF PEPTIDES AND GLYCOPEPTIDES

Waldmann, H., BRAUN, P., & KUNZ, H. (1991). NEW ENZYMATIC PROTECTING GROUP TECHNIQUES FOR THE CONSTRUCTION OF PEPTIDES AND GLYCOPEPTIDES. BIOMEDICA BIOCHIMICA ACTA, 50(10-11), S243-S248.

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 Creators:
Waldmann, Herbert1, Author           
BRAUN, P2, Author
KUNZ, H2, Author
Affiliations:
1Abt. IV: Chemische Biologie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753290              
2external, ou_persistent22              

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Free keywords: Biochemistry & Molecular Biology;
 Abstract: The use of non-proteases for the selective removal of protecting groups from peptides and glycopeptides is described. The N-terminal deprotection of peptides can be achieved by the hyrolysis of the phenylacetyl (PhAc) amide blocking group catalyzed by penicillin G acylase. On the other hand, the lipase-mediated hydrolysis of n-heptyl (Hep) and 2-bromoethyl esters allows for the liberation of the C-terminal carboxy group. The selective C-terminal deprotection can be applied advantageously for the construction of acid- and base-sensitive polyfunctional O-glycopeptides. In all cases the enzymatic reactions are completely selective and proceed under mildest conditions (pH 7-8, r.t. to 37-degrees-C) without damaging the various other functionalities present in the complex substrates.

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Language(s): eng - English
 Dates: 1991
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: A1991HC95400045
 Degree: -

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Title: BIOMEDICA BIOCHIMICA ACTA
Source Genre: Journal
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Publ. Info: BERLIN : AKADEMIE VERLAG
Pages: - Volume / Issue: 50 (10-11) Sequence Number: - Start / End Page: S243 - S248 Identifier: ISSN: 0232-766X