Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate

Martins, M. C. M., Hejazi, M., Fettke, J., Steup, M., Feil, R., Krause, U., et al. (2013). Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate. Plant physiology, 163(3), 1142-1163. doi:10.1104/pp.113.226787.

Item is

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Martins, Marina Camara Mattos1, Autor           
Hejazi, Mahdi2, Autor
Fettke, Joerg2, Autor
Steup, Martin2, Autor
Feil, R.1, Autor           
Krause, Ursula1, Autor           
Arrivault, S.1, Autor           
Vosloh, Daniel1, Autor           
Figueroa, Carlos Maria1, Autor           
Ivakov, A.3, Autor           
Yadav, Umesh Prasad1, Autor           
Piques, M.1, Autor           
Metzner, Daniela1, Autor           
Stitt, M.1, Autor           
Lunn, J. E.1, Autor           
Affiliations:
1System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
2external, ou_persistent22              
3Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (Arabidopsis thaliana) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 m in the cytosol, 0.2 to 0.5 m in the chloroplasts, and 0.05 m in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2013
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 24043444
DOI: 10.1104/pp.113.226787
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Plant physiology
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 163 (3) Artikelnummer: - Start- / Endseite: 1142 - 1163 Identifikator: ISSN: 1532-2548