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  Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate

Martins, M. C. M., Hejazi, M., Fettke, J., Steup, M., Feil, R., Krause, U., et al. (2013). Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate. Plant physiology, 163(3), 1142-1163. doi:10.1104/pp.113.226787.

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Martins, Marina Camara Mattos1, Author              
Hejazi, Mahdi2, Author
Fettke, Joerg2, Author
Steup, Martin2, Author
Feil, R.1, Author              
Krause, Ursula1, Author              
Arrivault, S.1, Author              
Vosloh, Daniel1, Author              
Figueroa, Carlos Maria1, Author              
Ivakov, A.3, Author              
Yadav, Umesh Prasad1, Author              
Piques, M.1, Author              
Metzner, Daniela1, Author              
Stitt, M.1, Author              
Lunn, J. E.1, Author              
Affiliations:
1System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
2external, ou_persistent22              
3Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              

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 Abstract: Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (Arabidopsis thaliana) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by beta-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 m in the cytosol, 0.2 to 0.5 m in the chloroplasts, and 0.05 m in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: 24043444
DOI: 10.1104/pp.113.226787
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Title: Plant physiology
Source Genre: Journal
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Pages: - Volume / Issue: 163 (3) Sequence Number: - Start / End Page: 1142 - 1163 Identifier: ISSN: 1532-2548