English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  L-edge X-ray absorption spectroscopy of dilute systems relevant to metalloproteins using an X-ray free-electron laser.

Mitzner, R., Rehanek, J., Kern, J., Gul, S., Hattne, J., Taguchi, T., et al. (2013). L-edge X-ray absorption spectroscopy of dilute systems relevant to metalloproteins using an X-ray free-electron laser. The Journal of Physical Chemistry Letters, 4(21), 3641-3647. doi:10.1021/jz401837f.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-A0D8-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CB7F-3
Genre: Journal Article

Files

show Files
hide Files
:
1854329.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
1854329.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1854329_Suppl_1.pdf (Supplementary material), 2MB
Name:
1854329_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://pubs.acs.org/doi/pdf/10.1021/jz401837f (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Mitzner, R., Author
Rehanek, J., Author
Kern, J., Author
Gul, S., Author
Hattne, J., Author
Taguchi, T., Author
Alonso-Mori, R., Author
Tran, R., Author
Weniger, C., Author
Schröder, H., Author
Quevedo, W., Author
Laksmono, H., Author
Sierra, R. G., Author
Han, G., Author
Lasalle-Kaiser, B., Author
Koroidov, S., Author
Kubicek, K., Author
Schreck, S., Author
Kunnus, K., Author
Brzhezinskaya, M., Author
Firsov, A., AuthorMinitti, M. P., AuthorTurner, J. J., AuthorMoseller, S., AuthorSauter, N. K., AuthorBogan, M. J., AuthorNordlund, D., AuthorSchlotter, W. F., AuthorMessinger, J., AuthorBorovik, A., AuthorTechert, S.1, Author              de Groot, F. M. F., AuthorFöhlisch, A., AuthorErko, A., AuthorBergmann, U., AuthorYachandra, V. K., AuthorWernet, P., AuthorYano, J., Author more..
Affiliations:
1Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for biophysical chemistry, Max Planck Society, ou_578564              

Content

show
hide
Free keywords: -
 Abstract: L-edge spectroscopy of 3d transition metals provides important electronic structure information and has been used in many fields. However, the use of this method for studying dilute aqueous systems, such as metalloenzymes, has not been prevalent because of severe radiation damage and the lack of suitable detection systems. Here we present spectra from a dilute Mn aqueous solution using a high-transmission zone-plate spectrometer at the Linac Coherent Light Source (LCLS). The spectrometer has been optimized for discriminating the Mn L-edge signal from the overwhelming O K-edge background that arises from water and protein itself, and the ultrashort LCLS X-ray pulses can outrun X-ray induced damage. We show that the deviations of the partial-fluorescence yield-detected spectra from the true absorption can be well modeled using the state-dependence of the fluorescence yield, and discuss implications for the application of our concept to biological samples.

Details

show
hide
Language(s): eng - English
 Dates: 2013-10-092013-11-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/jz401837f
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Physical Chemistry Letters
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 4 (21) Sequence Number: - Start / End Page: 3641 - 3647 Identifier: -