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  Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS

Araujo, W. L., Tohge, T., Nunes-Nesi, A., Obata, T., & Fernie, A. R. (2014). Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS. Methods in molecular biology, 1090, 107-119. doi:10.1007/978-1-62703-688-7_7.

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Araujo, W. L.1, Autor           
Tohge, T.1, Autor           
Nunes-Nesi, A.1, Autor           
Obata, T.1, Autor           
Fernie, A. R.1, Autor           
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Zusammenfassung: Plant metabolic pathways and the molecular and atomic fluxes through them can be deduced using stable isotopically labeled substrates. To this end one prerequisite is accurate measurement of the labeling pattern of targeted metabolites. Experiments are generally limited to the use of single-element isotopes, mainly (13)C. Here, we summarize the application of gas chromatography-time of flight mass spectrometry (GC-TOF-MS) for metabolic studies using differently labeled elemental isotopes applied to both intact organelles and whole plant tissue. This method allows quantitative evaluation of a broad range of metabolic pathways without the need for laborious (and potentially inaccurate) chemical fractionation procedures commonly used in the estimation of fluxes following incubation in radiolabeled substrates. We focus herein on the determination of isotope labeling in organic and amino acids.

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Sprache(n): eng - English
 Datum: 2014
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 24222412
DOI: 10.1007/978-1-62703-688-7_7
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Titel: Methods in molecular biology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 1090 Artikelnummer: - Start- / Endseite: 107 - 119 Identifikator: ISSN: 1940-6029