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  Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS

Araujo, W. L., Tohge, T., Nunes-Nesi, A., Obata, T., & Fernie, A. R. (2014). Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS. Methods in molecular biology, 1090, 107-119. doi:10.1007/978-1-62703-688-7_7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-B3B1-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-B3B2-D
Genre: Journal Article

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 Creators:
Araujo, W. L.1, Author              
Tohge, T.1, Author              
Nunes-Nesi, A.1, Author              
Obata, T.1, Author              
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Plant metabolic pathways and the molecular and atomic fluxes through them can be deduced using stable isotopically labeled substrates. To this end one prerequisite is accurate measurement of the labeling pattern of targeted metabolites. Experiments are generally limited to the use of single-element isotopes, mainly (13)C. Here, we summarize the application of gas chromatography-time of flight mass spectrometry (GC-TOF-MS) for metabolic studies using differently labeled elemental isotopes applied to both intact organelles and whole plant tissue. This method allows quantitative evaluation of a broad range of metabolic pathways without the need for laborious (and potentially inaccurate) chemical fractionation procedures commonly used in the estimation of fluxes following incubation in radiolabeled substrates. We focus herein on the determination of isotope labeling in organic and amino acids.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: ISI: 24222412
DOI: 10.1007/978-1-62703-688-7_7
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Title: Methods in molecular biology
Source Genre: Journal
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Pages: - Volume / Issue: 1090 Sequence Number: - Start / End Page: 107 - 119 Identifier: ISSN: 1940-6029