English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Blocking endocytosis enhances short-term synaptic depression under conditions of normal availability of vesicles.

Hua, Y., Woehler, A., Kahms, M., Haucke, V., Neher, E., & Klingauf, J. (2013). Blocking endocytosis enhances short-term synaptic depression under conditions of normal availability of vesicles. Neuron, 80(2), 343-349. doi:10.1016/j.neuron.2013.08.010.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-BB75-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1F69-B
Genre: Journal Article

Files

show Files
hide Files
:
1858982.pdf (Publisher version), 971KB
Name:
1858982.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1858982-PP.doc (Supplementary material), 272KB
Name:
1858982-PP.doc
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/msword / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1858982-Suppl.pdf (Supplementary material), 134KB
Name:
1858982-Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Hua, Y.1, Author              
Woehler, A.1, Author              
Kahms, M., Author
Haucke, V., Author
Neher, E.1, Author              
Klingauf, J., Author
Affiliations:
1Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1571137              

Content

show
hide
Free keywords: -
 Abstract: It is commonly thought that clathrin-mediated endocytosis is the rate-limiting step of synaptic transmission in small CNS boutons with limited capacity for synaptic vesicles, causing short-term depression during high rates of synaptic transmission. Here, we show by analyzing synaptopHluorin fluorescence that 200 action potentials evoke the same cumulative amount of vesicle fusion, irrespective of the frequency of stimulation (5–40 Hz), implying the absence of vesicle reuse, since the method used (alkaline-trapping) measures only first-round exocytosis. After blocking all slow or specifically clathrin-mediated endocytosis, however, the same stimulation patterns cause a rapid stimulation-frequency-dependent release depression. This form of depression does not reflect insufficient vesicle supply, but appears to be the result of slow clearance of vesicular components from the release site. Our findings uncover an important yet overlooked role of endocytic proteins for release site clearance in addition to their well-characterized role in endocytosis itsel

Details

show
hide
Language(s): eng - English
 Dates: 2013-10-16
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.neuron.2013.08.010
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Neuron
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 80 (2) Sequence Number: - Start / End Page: 343 - 349 Identifier: -