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  Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array.

Lim, J., Vrignon, J., Gruner, P., Karamitros, C. S., Konrad, M., & Baret, J. C. (2013). Ultra-high throughput detection of single cell beta-galactosidase activity in droplets using micro-optical lens array. Applied Physics Letters, 103(20): 203704. doi:10.1063/1.4830046.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-CEA9-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CB52-5
Genre: Journal Article

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1878012.pdf (Publisher version), 2MB
 
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Lim, J.1, Author              
Vrignon, J., Author
Gruner, P., Author
Karamitros, C. S.1, Author              
Konrad, M.1, Author              
Baret, J. C., Author
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1Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

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 Abstract: We demonstrate the use of a hybrid microfluidic-micro-optical system for the screening of enzymatic activity at the single cell level. Escherichia coli beta-galactosidase activity is revealed by a fluorogenic assay in 100 pl droplets. Individual droplets containing cells are screened by measuring their fluorescence signal using a high-speed camera. The measurement is parallelized over 100 channels equipped with microlenses and analyzed by image processing. A reinjection rate of 1 ml of emulsion per minute was reached corresponding to more than 10(5) droplets per second, an analytical throughput larger than those obtained using flow cytometry.

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Language(s): eng - English
 Dates: 2013-11-14
 Publication Status: Published online
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1063/1.4830046
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Title: Applied Physics Letters
Source Genre: Journal
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Pages: - Volume / Issue: 103 (20) Sequence Number: 203704 Start / End Page: - Identifier: -