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  Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell-like compartments

Jimenez, M., Martos, A., Cabre, E. J., Raso, A., & Rivas, G. (2013). Giant vesicles: a powerful tool to reconstruct bacterial division assemblies in cell-like compartments. ENVIRONMENTAL MICROBIOLOGY, 15(12, SI), 3158-3168. doi:10.1111/1462-2920.12214.

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 Creators:
Jimenez, Mercedes1, Author
Martos, Ariadna2, Author              
Cabre, Elisa J.1, Author
Raso, Ana2, Author              
Rivas, German1, Author
Affiliations:
1external, ou_persistent22              
2Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: PHYSIOLOGICAL IONIC-STRENGTH; UNILAMELLAR VESICLES; SYNTHETIC BIOLOGY; LIPOSOME MEMBRANES; ARTIFICIAL CELL; LIPID VESICLES; FTSZ RINGS; PROTEIN; RECONSTITUTION; ENCAPSULATION
 Abstract: The use of artificial lipid membranes, structured as giant unilamellar vesicles (GUVs), provides the opportunity to investigate membrane-associated biological processes under defined experimental conditions. Due to their large size, they are uniquely adapted to investigate the properties and organization (in time and space) of macromolecular complexes incorporated in the vesicle interior by imaging and micro-spectroscopic techniques. Experimental methods to produce giant vesicles and to encapsulate proteins inside them are here reviewed. Previous experimental work to reconstitute elements of the bacterial division machinery in these membrane-like systems is summarized. Future challenges towards reconstructing minimal divisome assemblies in giant vesicles as cytomimetic containers are discussed.

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Language(s): eng - English
 Dates: 2013-12
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000327711100004
DOI: 10.1111/1462-2920.12214
 Degree: -

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Title: ENVIRONMENTAL MICROBIOLOGY
  Alternative Title : Environ. Microbiol.
  Other : Special Issue: Reconstructing the essential bacterial cell cycle machinery.
Source Genre: Journal
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Publ. Info: 111 RIVER ST, HOBOKEN 07030-5774, NJ USA : WILEY-BLACKWELL
Pages: - Volume / Issue: 15 (12, SI) Sequence Number: - Start / End Page: 3158 - 3168 Identifier: ISSN: 1462-2912