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  Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails

Jasnin, M., Asano, S., Gouin, E., Hegerl, R., Plitzko, J. M., Villa, E., et al. (2013). Three-dimensional architecture of actin filaments in Listeria monocytogenes comet tails. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 110(51), 20521-20526. doi:10.1073/pnas.1320155110.

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 Creators:
Jasnin, Marion1, Author              
Asano, Shoh1, Author              
Gouin, Edith2, Author
Hegerl, Reiner1, Author              
Plitzko, Jürgen M.1, Author              
Villa, Elizabeth1, Author              
Cossart, Pascale2, Author
Baumeister, Wolfgang1, Author              
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1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: HOST-CELL ACTIN; ARP2/3 COMPLEX; ALPHA-ACTININ; F-ACTIN; OWN CYTOSKELETON; ATOMIC MODEL; PROTEIN; NUCLEATION; BUNDLES; MOVEMENTcellular actin structures; actin bundles; bundling proteins; force generation;
 Abstract: The intracellular bacterial pathogen Listeria monocytogenes is capable of remodelling the actin cytoskeleton of its host cells such that "comet tails" are assembled powering its movement within cells and enabling cell-to-cell spread. We used cryo-electron tomography to visualize the 3D structure of the comet tails in situ at the level of individual filaments. We have performed a quantitative analysis of their supramolecular architecture revealing the existence of bundles of nearly parallel hexagonally packed filaments with spacings of 12-13 nm. Similar configurations were observed in stress fibers and filopodia, suggesting that nanoscopic bundles are a generic feature of actin filament assemblies involved in motility; presumably, they provide the necessary stiffness. We propose a mechanism for the initiation of comet tail assembly and two scenarios that occur either independently or in concert for the ensuing actin-based motility, both emphasizing the role of filament bundling.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000328548600047
DOI: 10.1073/pnas.1320155110
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Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Source Genre: Journal
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Publ. Info: 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA : NATL ACAD SCIENCES
Pages: - Volume / Issue: 110 (51) Sequence Number: - Start / End Page: 20521 - 20526 Identifier: ISSN: 0027-8424