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  Towards whole-body fluorescence imaging in humans

Piper, S. K., Habermehl, C., Schmitz, C. H., Kuebler, W. M., Obrig, H., Steinbrink, J., et al. (2013). Towards whole-body fluorescence imaging in humans. PLoS One, 8(12): e83749. doi:10.1371/journal.pone.0083749.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-171E-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-86D0-A
Genre: Journal Article

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Piper_TowardsWholeBody.pdf (Publisher version), 2MB
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2013
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© 2013 Piper et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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 Creators:
Piper, Sophie K.1, 2, 3, Author
Habermehl, Christina1, 2, 3, Author
Schmitz, Christoph H.1, 4, Author
Kuebler, Wolfgang M.5, Author
Obrig, Hellmuth1, 6, 7, Author              
Steinbrink, Jens1, 3, Author
Mehnert, Jan1, 2, 3, Author              
Affiliations:
1Department of Neurology, Charité University Medicine Berlin, Germany, ou_persistent22              
2Department of Machine Learning, TU Berlin, Germany, ou_persistent22              
3Center for Stroke Research, Charité University Medicine Berlin, Germany, ou_persistent22              
4NIRx Medizintechnik GmbH, Berlin, Germany, ou_persistent22              
5Institute of Physiology, Charité University Medicine Berlin, Germany, ou_persistent22              
6Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634549              
7Clinic for Cognitive Neurology, University of Leipzig, Germany, ou_persistent22              

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 Abstract: Dynamic near-infrared fluorescence (DNIF) whole-body imaging of small animals has become a popular tool in experimental biomedical research. In humans, however, the field of view has been limited to body parts, such as rheumatoid hands, diabetic feet or sentinel lymph nodes. Here we present a new whole-body DNIF-system suitable for adult subjects. We explored whether this system (i) allows dynamic whole-body fluorescence imaging and (ii) can detect modulations in skin perfusion. The non-specific fluorescent probe indocyanine green (ICG) was injected intravenously into two subjects, and fluorescence images were obtained at 5 Hz. The in- and out-flow kinetics of ICG have been shown to correlate with tissue perfusion. To validate the system, skin perfusion was modulated by warming and cooling distinct areas on the chest and the abdomen. Movies of fluorescence images show a bolus passage first in the face, then in the chest, abdomen and finally in the periphery (,10, 15, 20 and 30 seconds, respectively). When skin perfusion is augmented by warming, bolus arrives about 5 seconds earlier than when the skin is cooled and perfusion decreased. Calculating bolus arrival times and spatial fitting of basis time courses extracted from different regions of interest allowed a mapping of local differences in subcutaneous skin perfusion. This experiment is the first to demonstrate the feasibility of whole-body dynamic fluorescence imaging in humans. Since the whole-body approach demonstrates sensitivity to circumscribed alterations in skinperfusion, it may be used to target autonomous changes in polyneuropathy and to screen for peripheral vascular diseases.

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Language(s): eng - English
 Dates: 2013-08-192013-11-072013-12-31
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: BibTex Citekey: piper2013towards
DOI: 10.1371/journal.pone.0083749
PMID: 24391820
PMC: PMC3877082
Other: eCollection 2013
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 8 (12) Sequence Number: e83749 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: /journals/resource/1000000000277850