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  Towards whole-body fluorescence imaging in humans

Piper, S. K., Habermehl, C., Schmitz, C. H., Kuebler, W. M., Obrig, H., Steinbrink, J., et al. (2013). Towards whole-body fluorescence imaging in humans. PLoS One, 8(12): e83749. doi:10.1371/journal.pone.0083749.

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Piper_TowardsWholeBody.pdf (Verlagsversion), 2MB
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Piper_TowardsWholeBody.pdf
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2013
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© 2013 Piper et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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 Urheber:
Piper, Sophie K.1, 2, 3, Autor
Habermehl, Christina1, 2, 3, Autor
Schmitz, Christoph H.1, 4, Autor
Kuebler, Wolfgang M.5, Autor
Obrig, Hellmuth1, 6, 7, Autor           
Steinbrink, Jens1, 3, Autor
Mehnert, Jan1, 2, 3, Autor           
Affiliations:
1Department of Neurology, Charité University Medicine Berlin, Germany, ou_persistent22              
2Department of Machine Learning, TU Berlin, Germany, ou_persistent22              
3Center for Stroke Research, Charité University Medicine Berlin, Germany, ou_persistent22              
4NIRx Medizintechnik GmbH, Berlin, Germany, ou_persistent22              
5Institute of Physiology, Charité University Medicine Berlin, Germany, ou_persistent22              
6Department Neurology, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634549              
7Clinic for Cognitive Neurology, University of Leipzig, Germany, ou_persistent22              

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 Zusammenfassung: Dynamic near-infrared fluorescence (DNIF) whole-body imaging of small animals has become a popular tool in experimental
biomedical research. In humans, however, the field of view has been limited to body parts, such as rheumatoid hands,
diabetic feet or sentinel lymph nodes. Here we present a new whole-body DNIF-system suitable for adult subjects. We explored whether this system (i) allows dynamic whole-body fluorescence imaging and (ii) can detect modulations in skin perfusion. The non-specific fluorescent probe indocyanine green (ICG) was injected intravenously into two subjects, and
fluorescence images were obtained at 5 Hz. The in- and out-flow kinetics of ICG have been shown to correlate with tissue
perfusion. To validate the system, skin perfusion was modulated by warming and cooling distinct areas on the chest and the
abdomen. Movies of fluorescence images show a bolus passage first in the face, then in the chest, abdomen and finally in the periphery (,10, 15, 20 and 30 seconds, respectively). When skin perfusion is augmented by warming, bolus arrives
about 5 seconds earlier than when the skin is cooled and perfusion decreased. Calculating bolus arrival times and spatial
fitting of basis time courses extracted from different regions of interest allowed a mapping of local differences in
subcutaneous skin perfusion. This experiment is the first to demonstrate the feasibility of whole-body dynamic fluorescence imaging in humans. Since the whole-body approach demonstrates sensitivity to circumscribed alterations in skinperfusion, it may be used to target autonomous changes in polyneuropathy and to screen for peripheral vascular diseases.

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Sprache(n): eng - English
 Datum: 2013-08-192013-11-072013-12-31
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: BibTex Citekey: piper2013towards
DOI: 10.1371/journal.pone.0083749
PMID: 24391820
PMC: PMC3877082
Anderer: eCollection 2013
 Art des Abschluß: -

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Titel: PLoS One
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: San Francisco, CA : Public Library of Science
Seiten: - Band / Heft: 8 (12) Artikelnummer: e83749 Start- / Endseite: - Identifikator: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850