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  Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system

Berger-Müller, S., Sugie, A., Takahashi, F., Tavosanis, G., Hakeda-Suzuki, S., & Suzuki, T. (2013). Assessing the role of cell-surface molecules in central synaptogenesis in the Drosophila visual system. PLoS One, 8(12): e83732. doi:10.1371/journal.pone.0083732.

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Berger-Müller, Sandra1, Author           
Sugie, Atsushi1, Author           
Takahashi, Fumio, Author
Tavosanis, Gaia, Author
Hakeda-Suzuki, Satoko1, Author           
Suzuki, Takashi1, Author           
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1Max Planck Research Group: Axonal Guidance and Neuronal Connectivity / Suzuki, MPI of Neurobiology, Max Planck Society, ou_1113554              

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Free keywords: SYNAPTIC-LAYER SPECIFICITY; PHOTORECEPTOR NEURONS; TRANSMEMBRANE PROTEIN; FUNCTIONAL DISSECTION; ADHESION MOLECULES; TARGET SPECIFICITY; BOUNDARY FORMATION; AXON; MELANOGASTER; RECEPTOR
 Abstract: A hallmark of the central nervous system is its spatial and functional organization in synaptic layers. During neuronal development, axons form transient contacts with potential post-synaptic elements and establish synapses with appropriate partners at specific layers. These processes are regulated by synaptic cell-adhesion molecules. In the Drosophila visual system, R7 and R8 photoreceptor subtypes target distinct layers and form en passant pre-synaptic terminals at stereotypic loci of the axonal shaft. A leucine-rich repeat transmembrane protein, Capricious (Caps), is known to be selectively expressed in R8 axons and their recipient layer, which led to the attractive hypothesis that Caps mediates R8 synaptic specificity by homophilic adhesion. Contradicting this assumption, our results indicate that Caps does not have a prominent role in synaptic-layer targeting and synapse formation in Drosophila photoreceptors, and that the specific recognition of the R8 target layer does not involve Caps homophilic axon-target interactions. We generated flies that express a tagged synaptic marker to evaluate the presence and localization of synapses in R7 and R8 photoreceptors. These genetic tools were used to assess how the synaptic profile is affected when axons are forced to target abnormal layers by expressing axon guidance molecules. When R7 axons were mistargeted to the R8-recipient layer, R7s either maintained an R7-like synaptic profile or acquired a similar profile to r8s depending on the overexpressed protein. When R7 axons were redirected to a more superficial medulla layer, the number of presynaptic terminals was reduced. These results indicate that cell-surface molecules are able to dictate synapse loci by changing the axon terminal identity in a partially cell-autonomous manner, but that presynapse formation at specific sites also requires complex interactions between pre- and post-synaptic elements.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Issued
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 8 (12) Sequence Number: e83732 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850