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  Intermediate dipolar distances from spin labels.

Marsh, D. (2014). Intermediate dipolar distances from spin labels. Journal of Magnetic Resonance, 238, 77-81. doi:10.1016/j.jmr.2013.11.001.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3677-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-EEFE-D
Genre: Journal Article

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 Creators:
Marsh, D.1, Author              
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1Emeritus Group of Spectroscopy and Photochemical Kinetics, MPI for Biophysical Chemistry, Max Planck Society, ou_578625              

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Free keywords: Inter-spin distances; Dipolar interactions; Second moment; Absolute first moment; Distance distribution; Spin label
 Abstract: Methods for determining inter-spin distances between nitroxide spin labels from dipolar couplings in the intermediate range (r(12) approximate to 1.1-2 nm) by CW-EPR are addressed. For nitroxide powder patterns, the assumption of unlike spins is a better approximation than assuming strong coupling between like spins. Methods that determine the average splitting in dipolar deconvolutions yield the mean value < 1/r(12)(3)>, and those that determine the dipolar contribution to the spectral second moment correspondingly yield < 1/r(12)(6)>. To relate these ensemble averages to the mean inter-spin distance < r(12)> requires knowledge of the distribution in r(12). Values of the inverse roots r(eff,n) = < 1/r(12)(n)>(-1/n) always lie below < r(12)>. Consistent comparisons of literature data on double-labelled alpha-helical peptides support this view. Evaluations of r(eff,n) for Gaussian distributions in r(12) yield calibrations to determine < r(12)> for a given distribution width, sigma(r12).

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Language(s): eng - English
 Dates: 2013-11-162014-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.jmr.2013.11.001
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Title: Journal of Magnetic Resonance
Source Genre: Journal
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Pages: - Volume / Issue: 238 Sequence Number: - Start / End Page: 77 - 81 Identifier: -