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  Atomic structure and handedness of the building block of a biological assembly.

Loquet, A., Habenstein, B., Chevelkov, V., Vasa, S. K., Giller, K., Becker, S., et al. (2013). Atomic structure and handedness of the building block of a biological assembly. Journal of the American Chemical Society, 135(51), 19135-19138. doi:10.1021/ja411362q.

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 Urheber:
Loquet, A.1, Autor           
Habenstein, B.1, Autor           
Chevelkov, V.1, Autor           
Vasa, S. K.2, Autor           
Giller, K.2, Autor           
Becker, S.2, Autor           
Lange, A.1, Autor           
Affiliations:
1Research Group of Solid-State NMR, MPI for biophysical chemistry, Max Planck Society, ou_persistent35              
2Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Zusammenfassung: Noncovalent supramolecular assemblies possess in general several unique subunit subunit interfaces.The basic building block of such an assembly consists of several subunits and contains all unique interfaces. Atomic-resolution structures of monomeric subunits are typically accessed by crystallography or solution NMR and fitted into electron microscopy density maps. However, the structure of the intact building block in the assembled state remains unknown with this hybrid approach. Here, we present the solid-state NMR atomic structure of the building block of the type III secretion system needle. The building block structure consists of a homotetrameric subunit complex with three unique supramolecular interfaces. Side-chain positions at the interfaces were solved at atomic detail. The high-resolution structure reveals unambiguously the helical handedness of the assembly, determined to be right-handed for the type III secretion system needle.Additionally, the axial rise per subunit could be extracted from the tetramer structure and independently validated by mass-per-length measurements.

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Sprache(n): eng - English
 Datum: 2013-12-132013-12-25
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1021/ja411362q
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Titel: Journal of the American Chemical Society
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 135 (51) Artikelnummer: - Start- / Endseite: 19135 - 19138 Identifikator: -