English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Atomic structure and handedness of the building block of a biological assembly.

Loquet, A., Habenstein, B., Chevelkov, V., Vasa, S. K., Giller, K., Becker, S., et al. (2013). Atomic structure and handedness of the building block of a biological assembly. Journal of the American Chemical Society, 135(51), 19135-19138. doi:10.1021/ja411362q.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-36A0-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3B04-F
Genre: Journal Article

Files

show Files
hide Files
:
1921620.pdf (Publisher version), 2MB
Name:
1921620.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1921620_Suppl_1.pdf (Supplementary material), 2MB
Name:
1921620_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://pubs.acs.org/doi/pdf/10.1021/ja411362q (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Loquet, A.1, Author              
Habenstein, B.1, Author              
Chevelkov, V.1, Author              
Vasa, S. K.2, Author              
Giller, K.2, Author              
Becker, S.2, Author              
Lange, A.1, Author              
Affiliations:
1Research Group of Solid-State NMR, MPI for biophysical chemistry, Max Planck Society, ou_persistent35              
2Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Content

show
hide
Free keywords: -
 Abstract: Noncovalent supramolecular assemblies possess in general several unique subunit subunit interfaces.The basic building block of such an assembly consists of several subunits and contains all unique interfaces. Atomic-resolution structures of monomeric subunits are typically accessed by crystallography or solution NMR and fitted into electron microscopy density maps. However, the structure of the intact building block in the assembled state remains unknown with this hybrid approach. Here, we present the solid-state NMR atomic structure of the building block of the type III secretion system needle. The building block structure consists of a homotetrameric subunit complex with three unique supramolecular interfaces. Side-chain positions at the interfaces were solved at atomic detail. The high-resolution structure reveals unambiguously the helical handedness of the assembly, determined to be right-handed for the type III secretion system needle.Additionally, the axial rise per subunit could be extracted from the tetramer structure and independently validated by mass-per-length measurements.

Details

show
hide
Language(s): eng - English
 Dates: 2013-12-132013-12-25
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/ja411362q
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of the American Chemical Society
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 135 (51) Sequence Number: - Start / End Page: 19135 - 19138 Identifier: -