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  Analysis of the Intestinal Microbiome of a Recovered Clostridium difficile Patient after Fecal Transplantation

Broecker, F., Kube, M., Klumpp, J., Schuppler, M., Biedermann, L., Hecht, J., et al. (2013). Analysis of the Intestinal Microbiome of a Recovered Clostridium difficile Patient after Fecal Transplantation. Digestion, 88(4), 243-251. doi:10.1159/000355955.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-38B8-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-80C7-C
Genre: Journal Article

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 Creators:
Broecker, Felix1, Author              
Kube, Michael, Author
Klumpp, Jochen, Author
Schuppler, Markus, Author
Biedermann, Luc, Author
Hecht, Jochen, Author
Hombach, Michael, Author
Keller, Peter M., Author
Rogler, Gerhard, Author
Moelling, Karin, Author
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1Chakkumal Anish, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863299              

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 Abstract: Background: Clostridium difficile infections upon antibiotic disruption of the gut microbiota are potentially lethal. Fecal microbiota transplantation (FMT) is a promising treatment option for recurrent C difficile-associated disease (CDAD). Here, we present a patient with recurrent CDAD that received FMT, leading to full recovery for what has now been 3 years. We performed metagenomic sequencing on stool samples to assess if there are indications for recolonization with C difficile and changes in the gut microbiota after FMT. Methods: DNA from the stool of the donor and recipient was subjected to illumina sequencing. Obtained read sets were assembled to contiguous sequences and open reading frames were predicted. Deduced proteins were taxonomically assigned. Results: We detected complex and apparently healthy nnicrobiomes in the donor's and recipient's intestines after FMT, but no indications for C. difficile colonization. Conclusions: Metagenomic analysis proved suitable to analyze the intestinal microbiome after FMT. Discussion of our evaluation procedure and data management may be helpful for future studies. We demonstrated restoration of a healthy and diverse gut microbiome with chimeric composition from donor and recipient, and long-lasting Clearance of C. difficile. The procedure is simple, cheap, caused no side effects, and was stable over 3 years. (C) 2013 S. Karger AG, Basel

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 Dates: 2013
 Publication Status: Published in print
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 Identifiers: ISI: 000329275800006
DOI: 10.1159/000355955
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Title: Digestion
Source Genre: Journal
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Publ. Info: Basel : S. Karger
Pages: - Volume / Issue: 88 (4) Sequence Number: - Start / End Page: 243 - 251 Identifier: ISSN: 0012-2823