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  Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II.

Meinel, D. M., Burkert-Kautzsch, C., Kieser, A., O'Duibhir, E., Siebert, M., Mayer, A., et al. (2013). Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II. PLoS Genetics, 9(11): e1003914. doi:10.1371/journal.pgen.1003914.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-36C8-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C4F6-B
Genre: Journal Article

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 Creators:
Meinel, D. M., Author
Burkert-Kautzsch, C., Author
Kieser, A., Author
O'Duibhir, E., Author
Siebert, M., Author
Mayer, A., Author
Cramer, P.1, Author              
Söding, J.2, Author              
Holstege, F. C., Author
Sträßer, K., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              
2Research Group of Computational Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1933286              

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 Abstract: Messenger RNA (mRNA) synthesis and export are tightly linked, but the molecular mechanisms of this coupling are largely unknown. In Saccharomyces cerevisiae, the conserved TREX complex couples transcription to mRNA export and mediates mRNP formation. Here, we show that TREX is recruited to the transcription machinery by direct interaction of its subcomplex THO with the serine 2-serine 5 (S2/S5) diphosphorylated CTD of RNA polymerase II. S2 and/or tyrosine 1 (Y1) phosphorylation of the CTD is required for TREX occupancy in vivo, establishing a second interaction platform necessary for TREX recruitment in addition to RNA. Genome-wide analyses show that the occupancy of THO and the TREX components Sub2 and Yra1 increases from the 59 to the 39 end of the gene in accordance with the CTD S2 phosphorylation pattern. Importantly, in a mutant strain, in which TREX is recruited to genes but does not increase towards the 39 end, the expression of long transcripts is specifically impaired. Thus, we show for the first time that a 59-39 increase of a protein complex is essential for correct expression of the genome. In summary, we provide insight into how the phospho-code of the CTD directs mRNP formation and export through TREX recruitment.

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Language(s): eng - English
 Dates: 2013-11-14
 Publication Status: Published online
 Pages: 15
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pgen.1003914
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Title: PLoS Genetics
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Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 9 (11) Sequence Number: e1003914 Start / End Page: - Identifier: ISSN: 1553-7390
CoNE: /journals/resource/1000000000017180