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  Global analysis of eukaryotic mRNA degradation reveals Xrn1-dependent buffering of transcript levels.

Sun, M., Schwalb, B., Pirkl, N., Maier, K. C., Schenk, A., Failmezger, H., et al. (2013). Global analysis of eukaryotic mRNA degradation reveals Xrn1-dependent buffering of transcript levels. Molecular cell, 52(1), 52-62. doi:10.1016/j.molcel.2013.09.010.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-36D5-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1D91-0
Genre: Journal Article

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 Creators:
Sun, M., Author
Schwalb, B., Author
Pirkl, N., Author
Maier, K. C., Author
Schenk, A., Author
Failmezger, H., Author
Tresch, A., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The rates of mRNA synthesis and degradation determine cellular mRNA levels and can be monitored by comparative dynamic transcriptome analysis (cDTA) that uses nonperturbing metabolic RNA labeling. Here we present cDTA data for 46 yeast strains lacking genes involved in mRNA degradation and metabolism. In these strains, changes in mRNA degradation rates are generally compensated by changes in mRNA synthesis rates, resulting in a buffering of mRNA levels. We show that buffering of mRNA levels requires the RNA exonuclease Xrn1. The buffering is rapidly established when mRNA synthesis is impaired, but is delayed when mRNA degradation is impaired, apparently due to Xrn1- dependent transcription repressor induction. Cluster analysis of the data defines the general mRNA degradation machinery, reveals different substrate preferences for the two mRNA deadenylase complexes Ccr4-Not and Pan2-Pan3, and unveils an interwoven cellular mRNA surveillance network.

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Language(s): eng - English
 Dates: 2013-10-10
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.molcel.2013.09.010
 Degree: -

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Title: Molecular cell
Source Genre: Journal
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Pages: - Volume / Issue: 52 (1) Sequence Number: - Start / End Page: 52 - 62 Identifier: -