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  The RNA polymerase trigger loop functions in all three phases of the transcription cycle.

Fouqueau, T., Zeller, M. E., Cheung, A. C., Cramer, P., & Thomm, M. (2013). The RNA polymerase trigger loop functions in all three phases of the transcription cycle. Nucleic acids research, 41(14), 7048-7059. doi:10.1093/nar/gkt433.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3798-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1EFD-9
Genre: Journal Article

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Fouqueau, T., Author
Zeller, M. E., Author
Cheung, A. C., Author
Cramer, P.1, Author              
Thomm, M., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The trigger loop (TL) forms a conserved element in the RNA polymerase active centre that functions in the elongation phase of transcription. Here, we show that the TL also functions in transcription initiation and termination. Using recombinant variants of RNA polymerase from Pyrococcus furiosus and a reconstituted transcription system, we demonstrate that the TL is essential for initial RNA synthesis until a complete DNA–RNA hybrid is formed. The archaeal TL is further important for transcription fidelity during nucleotide incorporation, but not for RNA cleavage during proofreading. A conserved glutamine residue in the TL binds the 2’-OH group of the nucleoside triphosphate (NTP) to discriminate NTPs from dNTPs. The TL also prevents aberrant transcription termination at non-terminator sites.

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Language(s): eng - English
 Dates: 2013-06-13
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gkt433
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Title: Nucleic acids research
Source Genre: Journal
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Pages: - Volume / Issue: 41 (14) Sequence Number: - Start / End Page: 7048 - 7059 Identifier: -