English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Kinetochore microtubule dynamics and attachment stability are regulated by Hec1

DeLuca, J. G., Gall, W. E., Ciferri, C., Cimini, D., Musacchio, A., & Salmon, E. D. (2006). Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. CELL, 127(5), 969-982. doi:10.1016/j.cell.2006.09.047.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
DeLuca, Jennifer G.1, Author
Gall, Walter E.1, Author
Ciferri, Claudio2, Author
Cimini, Daniela3, Author
Musacchio, Andrea4, Author           
Salmon, E. D.1, Author
Affiliations:
1Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA, ou_persistent22              
2Department of Experimental Oncology, European Institute of Oncology, Via Ripamonti 435, 20141 Milan, Italy, ou_persistent22              
3Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA, ou_persistent22              
4Abt. I:Mechanistische Zellbiologie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753287              

Content

show
hide
Free keywords: -
 Abstract: Mitotic cells face the challenging tasks of linking kinetochores to growing and, shortening microtubules and actively regulating these dynamic attachments to produce accurate chromosome segregation. We report here that Ndc80/Hec1 functions in regulating kinetochore microtubule plus-end dynamics and attachment stability. Microinjection of an antibody to the N terminus of Hec1 suppresses both microtubule detachment and microtubule plus-end polymerization and depolymerization at kinetochores of PtK1 cells. Centromeres become hyperstretched, kinetochore fibers shorten from spindle poles, kinetochore microtubule attachment errors increase, and chromosomes severely mis-segregate. The N terminus of Hec1 is phosphorylated by Aurora B kinase in vitro, and cells expressing N-terminal nonphosphorylatable mutants of Hec1 exhibit an increase in merotelic attachments, hyperstretching of centromeres, and errors in chromosome segregation. These findings reveal a key role for the Hec1 N terminus in controlling dynamic behavior of kinetochore microtubules.

Details

show
hide
Language(s):
 Dates: 2006
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: CELL
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 127 (5) Sequence Number: - Start / End Page: 969 - 982 Identifier: ISSN: 0092-8674