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  Photocrosslinking demonstrates proximity of a 34 kDa membrane protein to different portions of preprolactin during translocation through the endoplasmic reticulum.

Wiedmann, M., Görlich, D., Hartmann, E., Kurzchalia, T. V., & Rapoport, T. A. (1989). Photocrosslinking demonstrates proximity of a 34 kDa membrane protein to different portions of preprolactin during translocation through the endoplasmic reticulum. FEBS Letters, 257(2), 263-268. doi:10.1016/0014-5793(89)81549-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3BAC-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C4FC-0
Genre: Journal Article

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1922611.pdf (Publisher version), 649KB
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Wiedmann, M., Author
Görlich, D.1, Author              
Hartmann, E., Author
Kurzchalia, T. V., Author
Rapoport, T. A., Author
Affiliations:
1Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

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 Abstract: Photocrosslinking has been used to identify integral proteins of the endoplasmic reticulum membrane that are in proximity to nascent preprolactin during in vitro translocation. A photoreactive lysyl derivative was introduced into truncated preprolactin chains comprising 86 or 115 amino acids. Both with the 86mer, containing the reactive group in the signal sequence, and with the 115mer, containing the probe exclusively in the mature portion of the chain, photocrosslinking occurred to a 3̃5 kDa transmembrane glycoprotein, the signal sequence receptor (SSR). SSR is identical with a previously isolated abundant and ubiquitous 34 kDa membrane protein that appears to be essential for protein translocation.

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Language(s): eng - English
 Dates: 1989-11-06
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/0014-5793(89)81549-2
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 257 (2) Sequence Number: - Start / End Page: 263 - 268 Identifier: -