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  Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane-inserted signal sequence.

High, S., Martoglio, B., Görlich, D., Andersen, S. S., Ashford, A. J., Giner, A., et al. (1993). Site-specific photocross-linking reveals that Sec61p and TRAM contact different regions of a membrane-inserted signal sequence. The Journal of Biological Chemistry, 268(35), 26745-26751.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3BE2-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C643-F
Genre: Journal Article

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 Creators:
High, S., Author
Martoglio, B., Author
Görlich, D.1, Author              
Andersen, S. S., Author
Ashford, A. J., Author
Giner, A., Author
Hartmann, E., Author
Prehn, S., Author
Rapoport, T. A., Author
Dobberstein, B., Author
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1Department of Cellular Logistics, MPI for biophysical chemistry, Max Planck Society, ou_578574              

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 Abstract: A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This allowed the interactions of the NH2-terminal, the central, and the COOH-terminal regions of the signal sequence to be investigated during insertion into the membrane of the endoplasmic reticulum (ER). We found that different regions of the nascent chains were photocross-linked to different ER proteins. The TRAM protein (translocating chain-associating membrane protein) contacts the NH2-terminal region of the signal sequence while the mammalian Sec61p contacts the hydrophobic core of the signal sequence and regions COOH-terminal of this. These results suggest that the ER translocation complex is composed of heterologous protein subunits which contact distinct regions of nascent polypeptides during their membrane insertion.

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Language(s): eng - English
 Dates: 1993-12-15
 Publication Status: Published in print
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Title: The Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 268 (35) Sequence Number: - Start / End Page: 26745 - 26751 Identifier: -