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  A novel intermediate in transcription initiation by human mitochondrial RNA polymerase

Morozov, Y. I., Agaronyan, K., Cheung, A. C. M., Anikin, M., Cramer, P., & Temiakov, D. (2014). A novel intermediate in transcription initiation by human mitochondrial RNA polymerase. Nucleic Acids Research, 42(6), 3884-3893. doi:10.1093/nar/gkt1356.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3C0C-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CBB7-3
Genre: Journal Article

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 Creators:
Morozov, Y. I., Author
Agaronyan, K., Author
Cheung, A. C. M., Author
Anikin, M., Author
Cramer, P.1, Author              
Temiakov, D., Author
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The mitochondrial genome is transcribed by a single-subunit T7 phage-like RNA polymerase (mtRNAP), structurally unrelated to cellular RNAPs. In higher eukaryotes, mtRNAP requires two transcription factors for efficient initiation—TFAM, a major nucleoid protein, and TFB2M, a transient component of mtRNAP catalytic site. The mechanisms behind assembly of the mitochondrial transcription machinery and its regulation are poorly understood. We isolated and identified a previously unknown human mitochondrial transcription intermediate— a pre-initiation complex that includes mtRNAP, TFAM and promoter DNA. Using protein– protein cross-linking, we demonstrate that human TFAM binds to the N-terminal domain of mtRNAP, which results in bending of the promoter DNA around mtRNAP. The subsequent recruitment of TFB2M induces promoter melting and formation of an open initiation complex. Our data indicate that the pre-initiation complex is likely to be an important target for transcription regulation and provide basis for further structural, biochemical and biophysical studies of mitochondrial transcription.

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Language(s): eng - English
 Dates: 2014-01-062014-04
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gkt1356
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 42 (6) Sequence Number: - Start / End Page: 3884 - 3893 Identifier: -