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  Model of the Mediator middle module based on protein cross-linking

Larivière, L., Plaschka, C., Seizl, M., Petrotchenko, E. V., Wenzeck, L., Borchers, C. H., et al. (2013). Model of the Mediator middle module based on protein cross-linking. Nucleic Acids Research, 41(20), 9266-9273. doi:10.1093/nar/gkt704.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3C33-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C50B-8
Genre: Journal Article

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 Creators:
Larivière, L., Author
Plaschka, C., Author
Seizl, M., Author
Petrotchenko, E. V., Author
Wenzeck, L., Author
Borchers, C. H., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The essential core of the transcription coactivator Mediator consists of two conserved multiprotein modules, the head and middle modules. Whereas the structure of the head module is known, the structure of the middle module is lacking. Here we report a 3D model of a 6-subunit Mediator middle module. The model was obtained by arranging crystal structures and homology models of parts of the module based on lysine–lysine cross-links obtained by mass spectrometric analysis. The model contains a central tetramer formed by the heterodimers Med4/Med9 and Med7/Med21. The Med7/Med21 heterodimer is flanked by subunits Med10 and Med31. The model is highly extended, suggests that the middle module is flexible and contributes to a molecular basis for detailed structure–function studies of RNA polymerase II regulation.

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Language(s): eng - English
 Dates: 2013-08-11
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gkt704
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 41 (20) Sequence Number: - Start / End Page: 9266 - 9273 Identifier: -