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  Mediator phosphorylation prevents stress response transcription during non-stress conditions

Miller, C., Matic, I., Maier, K. C., Schwalb, B., Roether, S., Strässer, K., et al. (2012). Mediator phosphorylation prevents stress response transcription during non-stress conditions. Journal of Biological Chemistry, 287(53), 44017-44026. doi:10.1074/jbc.M112.430140.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3CA4-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3D85-A
Genre: Journal Article

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 Creators:
Miller, C., Author
Matic, I., Author
Maier, K. C., Author
Schwalb, B., Author
Roether, S., Author
Strässer, K., Author
Tresch, A., Author
Mann, M., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: Coactivator Transcription Gene Regulation Gene Transcription Mass Spectrometry (MS) Phosphorylation
 Abstract: The multiprotein complex Mediator is a coactivator of RNA polymerase (Pol) II transcription that is required for the regulated expression of protein-coding genes. Mediator serves as an end point of signaling pathways and regulates Pol II transcription, but the mechanisms it uses are not well understood. Here, we used mass spectrometry and dynamic transcriptome analysis to investigate a functional role of Mediator phosphorylation in gene expression. Affinity purification and mass spectrometry revealed that Mediator from the yeast Saccharomyces cerevisiae is phosphorylated at multiple sites of 17 of its 25 subunits. Mediator phosphorylation levels change upon an external stimulus set by exposure of cells to high salt concentrations. Phosphorylated sites in the Mediator tail subunit Med15 are required for suppression of stress-induced changes in gene expression under non-stress conditions. Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells.

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Language(s): eng - English
 Dates: 2012-11-07
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1074/jbc.M112.430140
 Degree: -

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Title: Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 287 (53) Sequence Number: - Start / End Page: 44017 - 44026 Identifier: -