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  CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II

Mayer, A., Heidemann, M., Lidschreiber, M., Schreieck, A., Sun, M., Hintermair, C., et al. (2012). CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II. Science, 336(6089), 1723-1725. doi:10.1126/science.1219651.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-3DF3-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-6959-B
Genre: Journal Article

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 Creators:
Mayer, A., Author
Heidemann, M., Author
Lidschreiber, M., Author
Schreieck, A., Author
Sun, M., Author
Hintermair, C., Author
Kremmer, E., Author
Eick, D., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: In different phases of the transcription cycle, RNA polymerase (Pol) II recruits various factors via its C-terminal domain (CTD), which consists of conserved heptapeptide repeats with the sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. We show that the CTD of transcribing yeast Pol II is phosphorylated at Tyr1, in addition to Ser2, Thr4, Ser5, and Ser7. Tyr1 phosphorylation stimulates binding of elongation factor Spt6 and impairs recruitment of termination factors Nrd1, Pcf11, and Rtt103. Tyr1 phosphorylation levels rise downstream of the transcription start site and decrease before the polyadenylation site, largely excluding termination factors from gene bodies. These results show that CTD modifications trigger and block factor recruitment and lead to an extended CTD code that explains transcription cycle coordination on the basis of differential phosphorylation of Tyr1, Ser2, and Ser5.

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Language(s): eng - English
 Dates: 2012-06-29
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.1219651
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 336 (6089) Sequence Number: - Start / End Page: 1723 - 1725 Identifier: -