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  A movie of the RNA polymerase nucleotide addition cycle.

Brueckner, F., Ortiz, J., & Cramer, P. (2009). A movie of the RNA polymerase nucleotide addition cycle. Current Opinion in Structural Biology, 19(3), 294-299. doi:10.1016/j.sbi.2009.04.005.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0015-7BBC-6 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0029-D7D0-6
Genre: Journal Article

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1935963.pdf (Publisher version), 479KB
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 Creators:
Brueckner, F., Author
Ortiz, J., Author
Cramer, P.1, Author           
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: During gene transcription, RNA polymerase (Pol) passes through repetitive cycles of adding a nucleotide to the growing mRNA chain. Here we obtained a movie of the nucleotide addition cycle by combining structural information on different functional states of the Pol II elongation complex (EC). The movie illustrates the two-step loading of the nucleoside triphosphate (NTP) substrate, closure of the active site for catalytic nucleotide incorporation, and the presumed two-step translocation of DNA and RNA, which is accompanied by coordinated conformational changes in the polymerase bridge helix and trigger loop. The movie facilitates teaching and a mechanistic analysis of transcription and can be downloaded from http://www.lmb.uni-muenchen.de/cramer/pr-materials.

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Language(s): eng - English
 Dates: 2009-06
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.sbi.2009.04.005
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Title: Current Opinion in Structural Biology
Source Genre: Journal
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Pages: - Volume / Issue: 19 (3) Sequence Number: - Start / End Page: 294 - 299 Identifier: -