English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regions.

Geiger, S. R., Kuhn, C. D., Leidig, C., Renkawitz, J., & Cramer, P. (2008). Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regions. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 64(5), 413-418. doi:10.1107/S174430910800972X.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-7C22-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C93C-7
Genre: Journal Article

Files

show Files
hide Files
:
1936009.pdf (Publisher version), 2MB
Name:
1936009.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Geiger, S. R., Author
Kuhn, C. D., Author
Leidig, C., Author
Renkawitz, J., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: multiprotein crystals; mapping unstructured regions; protein engineering; crystal engineering; RNA polymerase I; Rpb4/7 subcomplex
 Abstract: The removal of flexible protein regions is generally used to promote crystallization, but advanced strategies to quickly remove multiple flexible regions from proteins or protein complexes are lacking. Here, it is shown how a protein heterodimer with multiple flexibilities, the RNA polymerase I subcomplex A14/A43, could be crystallized with the use of an iterative procedure of predicting flexible regions, experimentally testing and improving these predictions and combining deletions of flexible regions in a stepwise manner. This strategy should enable the crystallization of other proteins and subcomplexes with multiple flexibilities, as required for hybrid structure solution of large macromolecular assemblies.

Details

show
hide
Language(s): eng - English
 Dates: 2008-06-282008-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S174430910800972X
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 64 (5) Sequence Number: - Start / End Page: 413 - 418 Identifier: -