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  Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells

Austen, K., Kluger, C., Freikamp, A., Chrostek-Grashoff, A., & Grashoff, C. (2013). Generation and Analysis of Biosensors to Measure Mechanical Forces Within Cells. In T. A. Baudino (Ed.), Methods in Molecular Biology, 1066 (pp. 169-184). New York: Springer.

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 Creators:
Austen, Katharina1, Author              
Kluger, Carleen1, Author              
Freikamp, Andrea1, Author              
Chrostek-Grashoff, Anna1, Author              
Grashoff, Carsten1, Author              
Affiliations:
1Grashoff, Carsten / Molecular Mechanotransduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565150              

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Free keywords: Mechanobiology , Mechanotransduction , Force measurement Biosensor , Tension sensor , Förster resonance energy transfer (FRET) , Fluorescence-lifetime imaging microscopy (FLIM)
 Abstract: The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-62703-604-7_15
 Degree: -

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Title: Methods in Molecular Biology, 1066
  Alternative Title : Cell-Cell Interactions: Methods and Protocols
Source Genre: Series
 Creator(s):
Baudino, Troy A., Editor
Affiliations:
-
Publ. Info: New York : Springer
Pages: - Volume / Issue: 1066 Sequence Number: - Start / End Page: 169 - 184 Identifier: ISBN: 978-1-62703-603-0