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  Archaeal transcription: Function of an alternative transcription Factor B from Pyrococcus furiosus.

Micorescu, M., Grünberg, S., Franke, A., Cramer, P., Thomm, M., & Bartlett, M. (2008). Archaeal transcription: Function of an alternative transcription Factor B from Pyrococcus furiosus. Journal of Bacteriology, 190(1), 157-167. doi:10.1128/JB.01498-07.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-8082-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C547-F
Genre: Journal Article

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1936520.pdf (Publisher version), 647KB
 
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 Creators:
Micorescu, M., Author
Grünberg, S., Author
Franke, A., Author
Cramer, P.1, Author              
Thomm, M., Author
Bartlett, M., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The genome of the hyperthermophile archaeon Pyrococcus furiosus encodes two transcription factor B (TFB) paralogs, one of which (TFB1) was previously characterized in transcription initiation. The second TFB (TFB2) is unusual in that it lacks recognizable homology to the archaeal TFB/eukaryotic TFIIB B-finger motif. TFB2 functions poorly in promoter-dependent transcription initiation, but photochemical cross-linking experiments indicated that the orientation and occupancy of transcription complexes formed with TFB2 at the strong gdh promoter are similar to the orientation and occupancy of transcription complexes formed with TFB1. Initiation complexes formed by TFB2 display a promoter opening defect that can be bypassed with a preformed transcription bubble, suggesting a mechanism to explain the low TFB2 transcription activity. Domain swaps between TFB1 and TFB2 showed that the low activity of TFB2 is determined mainly by its N terminus. The low activity of TFB2 in promoter opening and transcription can be partially relieved by transcription factor E (TFE). The results indicate that the TFB N-terminal region, containing conserved Zn ribbon and B-finger motifs, is important in promoter opening and that TFE can compensate for defects in the N terminus through enhancement of promoter opening.

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Language(s): eng - English
 Dates: 2007-10-262008-01
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1128/JB.01498-07
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Title: Journal of Bacteriology
Source Genre: Journal
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Pages: - Volume / Issue: 190 (1) Sequence Number: - Start / End Page: 157 - 167 Identifier: -