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  Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3

Hrle, A., Su, A. A. H., Ebert, J., Benda, C., Randau, L., & Conti, E. (2013). Structure and RNA-binding properties of the Type III-A CRISPR-associated protein Csm3. RNA BIOLOGY, 10(11), 1670-1678. doi:10.4161/rna.26500.

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 Creators:
Hrle, Ajla1, Author              
Su, Andreas A. H.2, Author
Ebert, Judith1, Author              
Benda, Christian1, Author              
Randau, Lennart2, Author
Conti, Elena1, Author              
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
2external, ou_persistent22              

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Free keywords: SHORT PALINDROMIC REPEATS; BACTERIAL IMMUNE-SYSTEM; PROCESSES PRE-CRRNA; CAS SYSTEMS; ADAPTIVE IMMUNITY; CRYSTAL-STRUCTURE; COMPLEX; RECOGNITION; DEFENSE; ARCHAEARAMP; RRM domain; ferredoxin domain; Cas7; adaptive immunity;
 Abstract: The prokaryotic adaptive immune system is based on the incorporation of genome fragments of invading viral genetic elements into clusters of regulatory interspaced short palindromic repeats (CRISPRs). The CRISPR loci are transcribed and processed into crRNAs, which are then used to target the invading nucleic acid for degradation. The large family of CRISPR-associated (Cas) proteins mediates this interference response. We have characterized Methanopyrus kandleri Csm3, a protein of the type III-A CRISPR-Cas complex. The 2.4 angstrom resolution crystal structure shows an elaborate four-domain fold organized around a core RRM-like domain. The overall architecture highlights the structural homology to Cas7, the Cas protein that forms the backbone of type I interference complexes. Csm3 binds unstructured RNAs in a sequence non-specific manner, suggesting that it interacts with the variable spacer sequence of the crRNA. The structural and biochemical data provide insights into the similarities and differences in this group of Cas proteins.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000330338200004
DOI: 10.4161/rna.26500
 Degree: -

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Title: RNA BIOLOGY
Source Genre: Journal
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Publ. Info: 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA : LANDES BIOSCIENCE
Pages: - Volume / Issue: 10 (11) Sequence Number: - Start / End Page: 1670 - 1678 Identifier: ISSN: 1547-6286