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  Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins.

Lavoie-Cardinal, F., Jensen, N. A., Westphal, V., Stiel, A. C., Chmyrov, A., Bierwagen, J., et al. (2014). Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins. ChemPhysChem, 15(4), 655-663. doi:10.1002/cphc.201301016.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-82CA-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-286E-B
Genre: Journal Article

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 Creators:
Lavoie-Cardinal, F.1, Author              
Jensen, N. A.1, Author              
Westphal, V.1, Author              
Stiel, A. C.1, Author              
Chmyrov, A.1, Author              
Bierwagen, J.1, Author              
Testa, I.1, Author              
Jakobs, S.2, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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Free keywords: luorescent proteins; nanoscopy; photoswitching; RESOLFT; superresolution imaging
 Abstract: Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single (“donut”) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23 000 “donut-like” minima are scanned simultaneously.

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Language(s): eng - English
 Dates: 2014-01-212014-03-17
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/cphc.201301016
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Title: ChemPhysChem
Source Genre: Journal
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Pages: - Volume / Issue: 15 (4) Sequence Number: - Start / End Page: 655 - 663 Identifier: -