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  Structure of an RNA polymerase II–RNA inhibitor complex elucidates transcription regulation by noncoding RNAs.

Kettenberger, H., Eisenführ, A., Brückner, F., Theis, M., Famulok, M., & Cramer, P. (2005). Structure of an RNA polymerase II–RNA inhibitor complex elucidates transcription regulation by noncoding RNAs. Nature Structural and Molecular Biology, 13(1), 44-48. doi:10.1038/nsmb1032.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-834F-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C55D-0
Genre: Journal Article

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1940296.pdf (Publisher version), 1MB
 
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 Creators:
Kettenberger, H., Author
Eisenführ, A., Author
Brückner, F., Author
Theis, M., Author
Famulok, M., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: The noncoding RNA B2 and the RNA aptamer FC bind RNA polymerase (Pol) II and inhibit messenger RNA transcription initiation, but not elongation. We report the crystal structure of FC*, the central part of FC RNA, bound to Pol II. FC* RNA forms a double stem-loop structure in the Pol II active center cleft. B2 RNA may bind similarly, as it competes with FC* RNA for Pol II interaction. Both RNA inhibitors apparently prevent the downstream DNA duplex and the template single strand from entering the cleft after DNA melting and thus interfere with open-complex formation. Elongation is not inhibited, as nucleic acids prebound in the cleft would exclude the RNA inhibitors. The structure also indicates that A-form RNA could interact with Pol II similarly to a B-form DNA promoter, as suggested for the bacterial transcription inhibitor 6S RNA.

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Language(s): eng - English
 Dates: 2005-12-112005
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsmb1032
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 13 (1) Sequence Number: - Start / End Page: 44 - 48 Identifier: -