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  Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors.

Meinhart, A., & Cramer, P. (2004). Recognition of RNA polymerase II carboxy-terminal domain by 3'-RNA-processing factors. Nature, 430(6996), 223-226. doi:10.1038/nature02679.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-850E-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C567-7
Genre: Journal Article

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 Creators:
Meinhart, A., Author
Cramer, P.1, Author              
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: During transcription, RNA polymerase (Pol) II synthesizes eukaryotic messenger RNA. Transcription is coupled to RNA processing by the carboxy-terminal domain (CTD) of Pol II, which consists of up to 52 repeats of the sequence Tyr 1-Ser 2-Pro 3-Thr 4-Ser 5-Pro 6-Ser 7 (refs 1, 2). After phosphorylation, the CTD binds tightly to a conserved CTD-interacting domain (CID) present in the proteins Pcf11 and Nrd1, which are essential and evolutionarily conserved factors for polyadenylation-dependent and -independent 3'-RNA processing, respectively. Here we describe the structure of a Ser 2-phosphorylated CTD peptide bound to the CID domain of Pcf11. The CTD motif Ser 2-Pro 3-Thr 4-Ser 5 forms a beta-turn that binds to a conserved groove in the CID domain. The Ser 2 phosphate group does not make direct contact with the CID domain, but may be recognized indirectly because it stabilizes the beta-turn with an additional hydrogen bond. Iteration of the peptide structure results in a compact beta-spiral model of the CTD. The model suggests that, during the mRNA transcription-processing cycle, compact spiral regions in the CTD are unravelled and regenerated in a phosphorylation-dependent manner.

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Language(s): eng - English
 Dates: 2004-07-08
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nature02679
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 430 (6996) Sequence Number: - Start / End Page: 223 - 226 Identifier: -