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  The mRNA transcription/processing factor Ssu72 Is a potential tyrosine phosphatase

Meinhart, A., Silberzahn, T., & Cramer, P. (2003). The mRNA transcription/processing factor Ssu72 Is a potential tyrosine phosphatase. Journal of Biological Chemistry, 278(18), 15917-15921. doi:10.1074/jbc.M301643200.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-86B6-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E404-F
Genre: Journal Article

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1940906.pdf (Publisher version), 659KB
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 Creators:
Meinhart, A., Author
Silberzahn, T., Author
Cramer, P.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: Ssu72 is an essential and highly conserved protein involved in mRNA transcription and 3-end processing. The biochemical function of Ssu72 was so far unknown. We report here evidence that Ssu72 is a phosphatase that resembles protein tyrosine phosphatases (PTPases). First, recombinant Ssu72 cleaves the phosphotyrosine analogue p-nitrophenylphosphate, and this catalytic activity is impaired by PTPase-inhibiting agents. Second, the Ssu72 sequence contains the CX5R signature motif of PTPases; mutation of the catalytic cysteine in this motif abolishes Ssu72 activity in vitro and has been shown to confer lethality in vivo. Third, secondary structure prediction and site-directed mutagenesis predict that Ssu72 adopts the fold of PTPases of the low molecular weight family. Distinguishing features, such as a short “aspartate loop” at the active site, suggest however that Ssu72 is the founding member of a new phosphatase subfamily. The novel Ssu72 activity may regulate coupling events during mRNA biogenesis.

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Language(s): eng - English
 Dates: 2003-02-262003-05-02
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1074/jbc.M301643200
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Title: Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 278 (18) Sequence Number: - Start / End Page: 15917 - 15921 Identifier: -