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  Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II

Bushnell, D. A., Cramer, P., & Kornberg, R. D. (2001). Selenomethionine incorporation in Saccharomyces cerevisiae RNA polymerase II. Structure, 9(1), R11-R14. doi:10.1016/S0969-2126(00)00554-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-8719-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-09A8-0
Genre: Journal Article

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1943331.pdf (Publisher version), 127KB
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Bushnell, D. A., Author
Cramer, P.1, Author              
Kornberg, R. D., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: A protocol for the incorporation of SeMet into yeast proteins is described. Incorporation at a level of about 50% suffices for the location of Se sites in an anomalous difference Fourier map of the 0.5 MDa yeast RNA polymerase II. This shows the utility of the approach as an aid in the model-building of large protein complexes.

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Language(s): eng - English
 Dates: 2001-01-01
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/S0969-2126(00)00554-2
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Title: Structure
Source Genre: Journal
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Pages: - Volume / Issue: 9 (1) Sequence Number: - Start / End Page: R11 - R14 Identifier: -