English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Structural basis of transcription: An RNA polymerase II elongation complex at 3.3 Å resolution

Gnatt, A. L., Cramer, P., Fu, J., Bushnell, D. A., & Kornberg, R. D. (2001). Structural basis of transcription: An RNA polymerase II elongation complex at 3.3 Å resolution. Science, 292(5523), 1876-1882. doi:10.1126/science.1059495.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-8723-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-696C-1
Genre: Journal Article

Files

show Files
hide Files
:
1943337.pdf (Publisher version), 824KB
Name:
1943337.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Gnatt, A. L., Author
Cramer, P.1, Author              
Fu, J., Author
Bushnell, D. A., Author
Kornberg, R. D., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: -
 Abstract: The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3  resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 39 end of the RNA in the nucleotide addition site. The 39 end is positioned above a pore, through which nucleotides may enter and through which RNA may be extruded during back-tracking. The 59-most residue of the RNA is close to the point of entry to an exit groove. Changes in protein structure between the transcribing complex and free enzyme include closure of a clamp over the DNA and RNA and ordering of a series of ÒswitchesÓ at the base of the clamp to create a binding site complementary to the DNA-RNA hybrid. ProteinÐ nucleic acid contacts help explain DNA and RNA strand separation, the speci Þcity of RNA synthesis, Òabortive cyclingÓ during transcription initiation, and RNA and DNA translocation during transcription elongation.

Details

show
hide
Language(s): eng - English
 Dates: 2001-06-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.1059495
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 292 (5523) Sequence Number: - Start / End Page: 1876 - 1882 Identifier: -