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  Architecture of RNA polymerase II and implications for the transcription mechanism

Cramer, P., Bushnell, D. A., Fu, J., Gnatt, A. L., Maier-Davis, B., Thompson, N. E., et al. (2000). Architecture of RNA polymerase II and implications for the transcription mechanism. Science, 288(5466), 640-649. doi:10.1126/science.288.5466.640.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-872F-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-6968-9
Genre: Journal Article

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 Creators:
Cramer, P.1, Author              
Bushnell, D. A., Author
Fu, J., Author
Gnatt, A. L., Author
Maier-Davis, B., Author
Thompson, N. E., Author
Burgess, R. R., Author
Edwards, A. M., Author
David, P. R., Author
Kornberg, R. D., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: A backbone model of a 10-subunit yeast RNA polymerase II has been derived from x-ray diffraction data extending to 3 angstroms resolution. All 10 subunits exhibit a high degree of identity with the corresponding human proteins, and 9 of the 10 subunits are conserved among the three eukaryotic RNA polymerases I, II, and III. Notable features of the model include a pair of jaws, formed by subunits Rpb1, Rpb5, and Rpb9, that appear to grip DNA downstream of the active center. A clamp on the DNA nearer the active center, formed by Rpb1, Rpb2, and Rpb6, may be locked in the closed position by RNA, accounting for the great stability of transcribing complexes. A pore in the protein complex beneath the active center may allow entry of substrates for polymerization and exit of the transcript during proofreading and passage through pause sites in the DNA.

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Language(s): eng - English
 Dates: 2000-04-28
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1126/science.288.5466.640
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Title: Science
Source Genre: Journal
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Pages: - Volume / Issue: 288 (5466) Sequence Number: - Start / End Page: 640 - 649 Identifier: -