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  Engineering of diffraction-quality crystals of the NF-KB P52 homodimer:DNA complex

Cramer, P., & Müller, C. W. (1997). Engineering of diffraction-quality crystals of the NF-KB P52 homodimer:DNA complex. FEBS Letters, 405(3), 373-377. doi:10.1016/S0014-5793(97)00217-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-8A2B-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-C57E-6
Genre: Journal Article

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1943897.pdf (Publisher version), 604KB
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 Creators:
Cramer, P.1, Author              
Müller, C. W., Author
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: Rel protein; NF-KB P52; Protein:DNA interaction; Co-crystallization; Transcription; Protein engineering
 Abstract: The eukaryotic transcription factors NF-KB P50 and NF-KB P52 are closely related members of the Rel family. Growth of diffraction-quality NF-KB P52:DNA co-crystals crucially depended on (a) extensive screens for the DNA fragment of optimal length and (b) engineering of the protein based on the two known NF-KB P50:DNA co-crystal structures [Miiller et al. (1995) Nature 373, 311-317; Ghosh et al. (1995) Nature 373, 303-310]; namely, deletion of 12 C-terminal amino acid residues. These residues are part of the Rel homology region and comprise the nuclear localization signal. The approach might be of general use for the crystallization of other Rel protein: DNA complexes and in our case yielded co-crystals which diffract beyond 2.0 A resolution.

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Language(s): eng - English
 Dates: 1997-04-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/S0014-5793(97)00217-2
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 405 (3) Sequence Number: - Start / End Page: 373 - 377 Identifier: -