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Free keywords:
Rel protein; NF-KB P52; Protein:DNA
interaction; Co-crystallization; Transcription; Protein
engineering
Abstract:
The eukaryotic transcription factors NF-KB P50 and
NF-KB P52 are closely related members of the Rel family.
Growth of diffraction-quality NF-KB P52:DNA co-crystals
crucially depended on (a) extensive screens for the DNA
fragment of optimal length and (b) engineering of the protein
based on the two known NF-KB P50:DNA co-crystal structures
[Miiller et al. (1995) Nature 373, 311-317; Ghosh et al. (1995)
Nature 373, 303-310]; namely, deletion of 12 C-terminal amino
acid residues. These residues are part of the Rel homology region
and comprise the nuclear localization signal. The approach might
be of general use for the crystallization of other Rel protein:
DNA complexes and in our case yielded co-crystals which
diffract beyond 2.0 A resolution.
