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  RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7.

Schreieck, A., Easter, A. D., Etzold, S., Wiederhold, K., Lidschreiber, M., Cramer, P., et al. (2014). RNA polymerase II termination involves C-terminal-domain tyrosine dephosphorylation by CPF subunit Glc7. Nature Structural and Molecular Biology, 21(2), 175-179. doi:10.1038/nsmb.2753.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0017-C302-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1DE1-D
Genre: Journal Article

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Schreieck, A., Author
Easter, A. D., Author
Etzold, S., Author
Wiederhold, K., Author
Lidschreiber, M., Author
Cramer, P.1, Author              
Passmore, L. A., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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 Abstract: At the 3' ends of protein-coding genes, RNA polymerase (Pol) II is dephosphorylated at tyrosine residues (Tyr1) of its C-terminal domain (CTD). In addition, the associated cleavage-and-polyadenylation factor (CPF) cleaves the transcript and adds a poly(A) tail. Whether these events are coordinated and how they lead to transcription termination remains poorly understood. Here we show that CPF from Saccharomyces cerevisiae is a Pol II-CTD phosphatase and that the CPF subunit Glc7 dephosphorylates Tyr1 in vitro. In vivo, the activity of Glc7 is required for normal Tyr1 dephosphorylation at the polyadenylation site, for recruitment of termination factors Pcf11 and Rtt103 and for normal Pol II termination. These results show that transcription termination involves Tyr1 dephosphorylation of the CTD and indicate that pre-mRNA processing by CPF and transcription termination are coupled via Glc7-dependent Pol II-Tyr1 dephosphorylation.

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Language(s): eng - English
 Dates: 2014-02
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nsmb.2753
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 21 (2) Sequence Number: - Start / End Page: 175 - 179 Identifier: -