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  Periodic mRNA synthesis and degradation co-operate during cell cycle gene expression.

Eser, P., Demel, C., Maier, K. C., Schwalb, B., Pirkl, N., Martin, D. E., et al. (2014). Periodic mRNA synthesis and degradation co-operate during cell cycle gene expression. Molecular Systems Biology, 10(1): 717. doi:10.1002/msb.134886.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0018-8B88-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC39-8
Genre: Journal Article

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 Creators:
Eser, P., Author
Demel, C., Author
Maier, K. C., Author
Schwalb, B., Author
Pirkl, N., Author
Martin, D. E., Author
Cramer, P.1, Author              
Tresch, A., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: cell cycle; gene regulation; mRNA degradation; mRNA turnover; periodic transcription
 Abstract: During the cell cycle, the levels of hundreds of mRNAs change in a periodic manner, but how this is achieved by alterations in the rates of mRNA synthesis and degradation has not been studied systematically. Here, we used metabolic RNA labeling and comparative dynamic transcriptome analysis (cDTA) to derive mRNA synthesis and degradation rates every 5 min during three cell cycle periods of the yeast Saccharomyces cerevisiae. A novel statistical model identified 479 genes that show periodic changes in mRNA synthesis and generally also periodic changes in their mRNA degradation rates. Peaks of mRNA degradation generally follow peaks of mRNA synthesis, resulting in sharp and high peaks of mRNA levels at defined times during the cell cycle. Whereas the timing of mRNA synthesis is set by upstream DNA motifs and their associated transcription factors (TFs), the synthesis rate of a periodically expressed gene is apparently set by its core promoter.

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Language(s): eng - English
 Dates: 2014-01-312014
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/msb.134886
 Degree: -

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Title: Molecular Systems Biology
Source Genre: Journal
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Pages: 15 Volume / Issue: 10 (1) Sequence Number: 717 Start / End Page: - Identifier: -