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  Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers.

Linser, R. (2012). Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers. Journal of Biomolecular NMR, 52(2), 151-158. doi:10.1007/s10858-011-9593-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0018-A850-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-80C2-0
Genre: Journal Article

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1977616.pdf (Publisher version), 3MB
 
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 Creators:
Linser, R.1, Author              
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1Research Group of Solid-State NMR-2, MPI for Biophysical Chemistry, Max Planck Society, ou_1950286              

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Free keywords: MAS solid-state NMR; Magic angle spinning; Perdeuteration; Proton detection; Backbone assignment; Cross polarization
 Abstract: For micro-crystalline proteins, solid-state nuclear magnetic resonance spectroscopy of perdeuterated samples can provide spectra of unprecedented quality. Apart from allowing to detect sparsely introduced protons and thereby increasing the effective resolution for a series of sophisticated techniques, deuteration can provide extraordinary coherence lifetimes--obtainable for all involved nuclei virtually without decoupling and enabling the use of scalar magnetization transfers. Unfortunately, for fibrillar or membrane-embedded proteins, significantly shorter transverse relaxation times have been encountered as compared to micro-crystalline proteins despite an identical sample preparation, calling for alternative strategies for resonance assignment. In this work we propose an approach towards sequential assignment of perdeuterated proteins based on long-range (1)H/(13)C Cross Polarization transfers. This strategy gives rise to H/N-separated correlations involving C(α), C(β), and CO chemical shifts of both, intra- and interresidual contacts, and thus connecting adjacent residues independent of transverse relaxation times.

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Language(s): eng - English
 Dates: 2011-12-142012-02
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10858-011-9593-2
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 52 (2) Sequence Number: - Start / End Page: 151 - 158 Identifier: -