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  Human stromal (mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential

Al-Nbaheen, M., Vishnubalaji, R., Ali, D., Bouslimi, A., Al-Jassir, F., Megges, M., et al. (2013). Human stromal (mesenchymal) stem cells from bone marrow, adipose tissue and skin exhibit differences in molecular phenotype and differentiation potential. Stem Cell Reviews, 9(1), 32-43. doi:10.1007/s12015-012-9365-8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-4380-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-4381-B
Genre: Journal Article

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The Author(s) 2012. This article is published with open access at Springerlink.com
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 Creators:
Al-Nbaheen, M., Author
Vishnubalaji, R., Author
Ali, D., Author
Bouslimi, A., Author
Al-Jassir, F., Author
Megges, M.1, Author              
Prigione, A.1, Author              
Adjaye, J.1, Author              
Kassem, M., Author
Aldahmash, A., Author
Affiliations:
1Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479654              

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Free keywords: Adipocytes/cytology/metabolism Adipose Tissue/*cytology Alkaline Phosphatase/analysis Antigens, CD/biosynthesis Biological Markers Bone Marrow Bone Marrow Cells/*cytology Cell Culture Techniques Cell Differentiation Cell Proliferation Cells, Cultured Humans Male Mesenchymal Stromal Cells/*cytology Oligonucleotide Array Sequence Analysis Osteoblasts/metabolism Phenotype Skin/*cytology
 Abstract: Human stromal (mesenchymal) stem cells (hMSCs) are multipotent stem cells with ability to differentiate into mesoderm-type cells e.g. osteoblasts and adipocytes and thus they are being introduced into clinical trials for tissue regeneration. Traditionally, hMSCs have been isolated from bone marrow, but the number of cells obtained is limited. Here, we compared the MSC-like cell populations, obtained from alternative sources for MSC: adipose tissue and skin, with the standard phenotype of human bone marrow MSC (BM-MSCs). MSC from human adipose tissue (human adipose stromal cells (hATSCs)) and human skin (human adult skin stromal cells, (hASSCs) and human new-born skin stromal cells (hNSSCs)) grew readily in culture and the growth rate was highest in hNSSCs and lowest in hATSCs. Compared with phenotype of hBM-MSC, all cell populations were CD34(-), CD45(-), CD14(-), CD31(-), HLA-DR(-), CD13(+), CD29(+), CD44(+), CD73(+), CD90(+),and CD105(+). When exposed to in vitro differentiation, hATSCs, hASSCs and hNSSCs exhibited quantitative differences in their ability to differentiate into adipocytes and to osteoblastic cells. Using a microarray-based approach we have unveiled a common MSC molecular signature composed of 33 CD markers including known MSC markers and several novel markers e.g. CD165, CD276, and CD82. However, significant differences in the molecular phenotype between these different stromal cell populations were observed suggesting ontological and functional differences. In conclusion, MSC populations obtained from different tissues exhibit significant differences in their proliferation, differentiation and molecular phenotype, which should be taken into consideration when planning their use in clinical protocols.

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Language(s): eng - English
 Dates: 2013-02-012013
 Publication Status: Published in print
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Title: Stem Cell Reviews
  Alternative Title : Stem cell reviews
Source Genre: Journal
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Publ. Info: Springer
Pages: - Volume / Issue: 9 (1) Sequence Number: - Start / End Page: 32 - 43 Identifier: -